Abstract

Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Curabitur sodales ligula in libero. Sed dignissim lacinia nunc. Curabitur tortor. Pellentesque nibh. Aenean quam. In scelerisque sem at dolor. Maecenas mattis. Sed convallis tristique sem. Proin ut ligula vel nunc egestas porttitor. Morbi lectus risus, iaculis vel, suscipit quis, luctus non, massa. Fusce ac turpis quis ligula lacinia aliquet. Mauris ipsum. Nulla metus metus, ullamcorper vel, tincidunt sed, euismod in, nibh. Quisque volutpat condimentum velit. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Nam nec ante. Sed lacinia, urna non tincidunt mattis, tortor neque adipiscing diam, a cursus ipsum ante quis turpis. Nulla facilisi Ut fringilla. Suspendisse potenti Nunc feugiat mi a tellus consequat imperdiet. Vestibulum sapien. Proin quam. Etiam ultrices. Suspendisse in justo eu magna luctus suscipit. Sed lectus. Integer euismod lacus luctus magna. Quisque cursus, metus vitae pharetra auctor, sem massa mattis sem, at interdum magna augue eget diam.

KeyWords: Lorem, ipsum, dolor, sit amet, consectetur.

1 Introduction

Lorem ipsum (Abbai et al., 2019) dolor sit amet, consectetur adipiscing elit. Integer nec odio. Praesent libero. Sed cursus ante dapibus diam. Sed nisi. Nulla quis sem at nibh elementum imperdiet. Duis sagittis ipsum. Praesent mauris Fusce nec tellus sed augue semper porta. Mauris massa. Vestibulum lacinia arcu eget nulla Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Curabitur sodales ligula in libero. Sed dignissim lacinia nunc (Bergström et al., 2020; Bhatt et al., 2019).

Curabitur tortor (Donald & Hamblin, 1976) Pellentesque nibh. Aenean quam. In scelerisque sem at dolor. Maecenas mattis. Sed convallis tristique sem. Proin ut ligula vel nunc egestas porttitor. Morbi lectus risus, iaculis vel, suscipit quis, luctus non, massa. Fusce ac turpis quis ligula lacinia aliquet. Mauris ipsum. Nulla metus metus, ullamcorper vel, tincidunt sed, euismod in, nibh. Quisque volutpat condimentum velit. Nulla facilisi. Ut fringilla. Suspendisse potenti. Nunc feugiat mi a tellus consequat imperdiet. Vestibulum sapien. Proin quam. Etiam ultrice. Suspendisse in justo eu magna luctus suscipit. Sed lectus. Integer euismod lacus luctus magna.

2 Materials and Methods

The data was analyzed in the statistical software R (R Core Team, 2020). The germination analysis and graphics was carried out with the package GerminaR (Lozano Isla et al., 2020). Each variable was submitted at analysis of variance (ANOVA) and the mean comparison test used was Student-Newman Keuls (P<0.05) (de Mendiburu, 2020). For the multivariate analysis, the principal components analysis (PCA) and cluster hierarchical classification analysis (HCPC) will be used .

The vertical bars represent the means (±SE). The mean differences between the groups are represented by different capital letters and into the group different lowercase letters (SNK, p = 0.05) (Husson et al., 2020).

More information about multivarite analysis in http://factominer.free.fr/index.html. Explanation for intepretation and analysis:

PCA: https://www.youtube.com/watch?v=pks8m2ka7Pk&feature=youtu.be

HCPC: https://www.youtube.com/watch?v=4XrgWmN9erg

2.1 Data set

The data set used for this analysis is available in the following link: DATA SET

2.2 Nulla metus metus

Curabitur tortor. Pellentesque nibh. Aenean quam. In scelerisque sem at dolor. Maecenas mattis. Sed convallis tristique sem. Proin ut ligula vel nunc egestas porttitor. Morbi lectus risus, iaculis vel, suscipit quis, luctus non, massa. Fusce ac turpis quis ligula lacinia aliquet. Mauris ipsum. Nulla metus metus, ullamcorper vel, tincidunt sed, euismod in, nibh. Quisque volutpat condimentum velit. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Nam nec ante (Table 7.1).

3 Results

3.1 Sed convallis tristique sem

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Integer nec odio. Praesent libero. Sed cursus ante dapibus diam. Sed nisi. Nulla quis sem at nibh elementum imperdiet. Duis sagittis ipsum. Praesent mauris. Fusce nec tellus sed augue semper porta. Mauris massa. Vestibulum lacinia arcu eget nulla. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos \(g=\left(\frac{\sum_{i=1}^kn_1}{N}\right)100\).

Curabitur sodales ligula in libero. Sed dignissim lacinia nunc. Curabitur tortor. Pellentesque nibh. Aenean quam. In scelerisque sem at dolor. Maecenas mattis. Sed convallis tristique sem. Proin ut ligula vel nunc egestas porttitor. Morbi lectus risus, iaculis vel, suscipit quis, luctus non, massa. Fusce ac turpis quis ligula lacinia aliquet Mauris ipsum .

# load data

library(GerminaR) 

fb <- prosopis %>% 
  dplyr::mutate(across(c(rep, nacl, temp),  ~ as.character(.)))

# germination analysis

gsm <- ger_summary(SeedN = "seeds", evalName = "D", data = fb)
str(gsm)

# analisys of variance

av <- aov(formula = grp ~ nacl*temp + rep, data = gsm)
summary(av)

# mean comparision test

mc <- ger_testcomp(aov = av, comp = c("temp", "nacl"), type = "snk")

3.2 Class aptent taciti

Nulla metus metus, ullamcorper vel, tincidunt sed, euismod in, nibh. Quisque volutpat condimentum velit. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Nam nec ante. Sed lacinia, urna non tincidunt mattis, tortor neque adipiscing diam, a cursus ipsum ante quis turpis. Nulla facilisi. Ut fringilla. Suspendisse potenti. Nunc feugiat mi a tellus consequat imperdiet. Vestibulum sapien. Proin quam. Etiam ultrices (Figure 7.2).

4 Discussion

Curabitur tortor (Lozano-Isla et al., 2019), Pellentesque nibh. Aenean quam. In scelerisque sem at dolor. Maecenas mattis. Sed convallis tristique sem. Proin ut ligula vel nunc egestas porttitor. Morbi lectus risus, iaculis vel, suscipit quis, luctus non, massa. Fusce ac turpis quis ligula lacinia aliquet. Mauris ipsum. Nulla metus metus, ullamcorper vel, tincidunt sed, euismod in, nibh. Quisque volutpat condimentum velit. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Nam nec ante (Figure 7.1).

Sed lacinia, urna non tincidunt mattis, tortor neque adipiscing diam, a cursus ipsum ante quis turpis. Nulla facilisi. Ut fringilla. Suspendisse potenti. Nunc feugiat mi a tellus consequat imperdiet. Vestibulum sapien. Proin quam. Etiam ultrices. Suspendisse in justo eu magna luctus suscipit. Sed lectus. Integer euismod lacus luctus magna. Quisque cursus, metus vitae pharetra auctor, sem massa mattis sem, at interdum magna augue eget diam. Vestibulum ante ipsum primis in faucibus orci luctus et ultrices posuere cubilia Curae; Morbi lacinia molestie dui. Praesent blandit dolor .

5 Conclusions

Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Curabitur sodales ligula in libero. Sed dignissim lacinia nunc. Curabitur tortor. Pellentesque nibh. Aenean quam. In scelerisque sem at dolor. Maecenas mattis. Sed convallis tristique sem. Proin ut ligula vel nunc egestas porttitor. Morbi lectus risus, iaculis vel, suscipit quis, luctus non, massa. Fusce ac turpis quis ligula lacinia aliquet. Mauris ipsum. Nulla metus metus, ullamcorper vel, tincidunt sed, euismod in, nibh. Quisque volutpat condimentum velit. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Nam nec ante. Sed lacinia, urna non tincidunt mattis, tortor neque adipiscing diam, a cursus ipsum ante quis turpis. Nulla facilisi. Ut fringilla. Suspendisse potenti. Nunc feugiat mi a tellus consequat imperdiet.

6 Acknowledgments

Donec lacus nunc, viverra nec, blandit vel, egestas et, augue. Vestibulum tincidunt malesuada tellus. Ut ultrices ultrices enim. Curabitur sit amet mauris. Morbi in dui quis est pulvinar ullamcorper. Nulla facilisi. Integer lacinia sollicitudin massa. Cras metus. Sed aliquet risus a tortor. Integer id quam. Morbi mi.

7 References

Abbai, R., Singh, V. K., Nachimuthu, V. V., Sinha, P., Selvaraj, R., Vipparla, A. K., Singh, A. K., Singh, U. M., Varshney, R. K., & Kumar, A. (2019). Haplotype analysis of key genes governing grain yield and quality traits across 3K rg panel reveals scope for the development of tailor-made rice with enhanced genetic gains. Plant Biotechnology Journal, 17(8), 1612–1622. https://doi.org/10.1111/pbi.13087

Bergström, A., McCarthy, S. A., Hui, R., Almarri, M. A., Ayub, Q., Danecek, P., Chen, Y., Felkel, S., Hallast, P., Kamm, J., & al. (2020). Insights into human genetic variation and population history from 929 diverse genomes. Science, 367(6484). https://doi.org/10.1126/science.aay5012

Bhatt, A., Bhat, N. R., Lozano-Isla, F., Gallacher, D., Santo, A., Batista-Silva, W., Fernandes, D., & Pompelli, M. F. (2019). Germination asynchrony is increased by dual seed bank presence in two desert perennial halophytes. Botany, 97(11), 639–649. https://doi.org/10.1139/cjb-2019-0071

de Mendiburu, F. (2020). Agricolae: Statistical procedures for agricultural research. https://CRAN.R-project.org/package=agricolae

Donald, C. M., & Hamblin, J. (1976). The biological yield and harvest index of cereals as agronomic and plant breeding criteria. In N. C. Brady (Ed.), Advances in agronomy (Vol. 28, pp. 361–405). Academic Press. http://www.sciencedirect.com/science/article/pii/S0065211308605593

Husson, F., Josse, J., Le, S., & Mazet, J. (2020). FactoMineR: Multivariate exploratory data analysis and data mining. https://CRAN.R-project.org/package=FactoMineR

Lozano Isla, F., Benites Alfaro, O., Garcia de Santana, D., Ranal, M. A., & Pompelli, M. F. (2020). GerminaR: Indices and graphics for assess seed germination process. https://flavjack.github.io/germinaquant/

Lozano-Isla, Flavio, Benites-Alfaro, Omar Eduardo, Pompelli, & Marcelo Francisco. (2019). GerminaR: An R package for germination analysis with the interactive web application “GerminaQuant for R”. Ecological Research, 34(2), 339–346. https://doi.org/10.1111/1440-1703.1275

R Core Team. (2020). R: A language and environment for statistical computing. R Foundation for Statistical Computing. https://www.R-project.org/

Schafleitner, R., Gutierrez, R., Espino, R., Gaudin, A., Pérez, J., Martínez, M., Domínguez, A., Tincopa, L., Alvarado, C., Numberto, G., & al. (2007). Field screening for variation of drought tolerance in solanum tuberosum l. By agronomical, physiological and genetic analysis. Potato Research, 50(1), 71–85. https://doi.org/10.1007/s11540-007-9030-9

Tables

Table 7.1: Germination dataset import from a googlesheet
rep	nacl	temp	seeds	D1	D2	D3	D4
1	0.0	25	50	39	8	3	0
2	0.0	25	50	40	9	1	0
3	0.0	25	50	34	16	0	0
4	0.0	25	50	43	7	0	0
1	0.0	30	50	48	2	0	0
2	0.0	30	50	47	3	0	0
3	0.0	30	50	50	0	0	0
4	0.0	30	50	49	1	0	0
1	0.5	25	50	10	37	1	2
2	0.5	25	50	18	30	1	1

Source: ^a Schafleitner et al. (2007)

Figures

Germination experiment with *Prosopis juliflor* under different osmotic potentials and temperatures. __Where:__ A) Bar graph with germination percentage in a factorial analisys. B) Line graph from cumulative germination under different osmotic potentials.

Figure 7.1: Germination experiment with Prosopis juliflor under different osmotic potentials and temperatures. Where: A) Bar graph with germination percentage in a factorial analisys. B) Line graph from cumulative germination under different osmotic potentials.

Figure 7.2: Plant of Jatropha curcas. A) Foliage, B) Leaf, C) Fruit.

Figure 7.3: Multivariate Analysis: Principal component Analysis and Hierarchical Clustering Analysis.

Rticles: Scientific Documents with Bookdown R Package

www.lozanoisla.com

Jimmy Gomez \(^{1}\), Flavio Lozano-Isla \(^{2*}\)

\(^{1}\) Agronomist, Co-founder ww.quipo.org

\(^{2}\) Agronomist MSc, email: flavjack@gmail.com

2020-08-09 08:10